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In response to growing public concern and philosophical debate, governments have begun to enact legislation to protect animal welfare. In the United States, the Animal Welfare Act of 1966 was a significant milestone, providing federal guidelines for the treatment of animals in research, exhibition, and transportation. Similarly, the European Union's Directive 2010/63/EU established strict regulations for animal research, while the UK's Animal Welfare Act 2006 strengthened protections for animals in various contexts.